This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational "from bed to bench and back" studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
| ISBN: | 9781493994236 |
| Publication date: | 7th June 2019 |
| Author: | Ulla G. Knaus, Thomas L. Leto |
| Publisher: | Humana Press an imprint of Springer New York |
| Format: | Hardback |
| Pagination: | 718 pages |
| Series: | Methods in Molecular Biology |
| Genres: |
Chemical biology Physiology Medical genetics |