This book reflects the drug development community's interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silico lead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
| ISBN: | 9781493972005 |
| Publication date: | 15th August 2017 |
| Author: | Iulia M. Lazar, Maria Kontoyianni, Alexandru C. Lazar |
| Publisher: | Humana Press an imprint of Springer New York |
| Format: | Hardback |
| Pagination: | 302 pages |
| Series: | Methods in Molecular Biology |
| Genres: |
Pharmacology |