This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. Chapter “Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
ISBN: | 9781071627068 |
Publication date: | 31st August 2022 |
Author: | Kiyotake Ishikawa |
Publisher: | Springer-Verlag New York Inc. |
Format: | Hardback |
Pagination: | 347 pages |
Series: | Methods in Molecular Biology |
Genres: |
Genetics (non-medical) Cardiovascular medicine Medical genetics |